文章基本信息

标题：Growth Pattern and Copper Accumulation in Callus of Datura metel
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作者：Yulita Nurchayati ; Yulita Nurchayati ; Yulita Nurchayati 等
期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2016
卷号：8
期号：2
页码：135-140
DOI：10.15294/biosaintifika.v8i2.5177
语种：English
出版社：Universitas Negeri Semarang
摘要：This experiment was aimed to evaluate the copper accumulation using callus culture of Datura metel L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl2 .2H2 O and Na2 CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 μM. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20th doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl2 .2H2 O at 5-15 μM of level concentration but declined at 20μM. The maximum rate of accumulation of Cu (0,1519 mg g-1) was obtained at 15 μM. Instead, the addition of Na2 CuEDTA at 5-20μM of level concentration showed the significant increment while the maximum accumulation was obtained at 20μM (0,1420 mg g-1). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of D. metel as that fairly absorbed and accumulated Cu2+. Exposure with CuCl2 .2H2 O indicated higher accumulation than Na2 CuEDTA.
关键词：Datura metel L; callus culture; Copper; Bioaccumulation; Phytoremediation