文章基本信息

标题：Proteases from Latex of Euphorbia spp. and Its Application on Milk Clot Formation
其他标题：Protease dari Latex Euphorbia spp. dan Aplikasinya dalam Penggumpalan Susu
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作者：Fidia Fibriana ; Fidia Fibriana ; Apichat Upaichit 等
期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2015
卷号：7
期号：2
页码：92-99
DOI：10.15294/biosaintifika.v7i2.3951
语种：English
出版社：Universitas Negeri Semarang
摘要：Crude proteases were extracted from Euphorbiaceae family, i.e. E. milii var imperata, E. trigona, and E. maculata. Among those three crude proteases, the activity of protease from E. trigona was the highest (812.50 U/ml), whereas E. milii and E. maculata crude proteases activity were 298.60 U/ml and 95.80 U/ml, respectively. E. maculata protein concentration was the highest among those three crude enzymes (1.206 mg/ml). The optimum pH and temperature of the enzymes were pH 7.0, pH 6.0, pH 6.5 and 60 °C, 50 °C, and 50 °C, respectively. Crude protease from E. milii var imperata, E. trigona, and E. maculata retained proteolytic activity over a wide range of pH (5.0–9.0) and temperature (up to 65 °C) with casein as substrate. All crude proteases showed milk clotting activity ranged from 0.58 U/ml to 1.01 U/ml. Thus, these crude proteases are potential to be applied in dairy industries. However, further study on enzyme purification and characterization are necessary to obtain high purity of proteases before its application.
其他摘要：Protease kasar berhasil diekstrak dari tanaman family Euphorbiaceae, yaitu E. milii var imperata, E. trigona, dan E. maculata. Diantara ketiga protease tersebut, aktivitas protease tertinggi diperoleh dari E. trigona (812,50 U/ml), sedangkan aktivitas pro
关键词：plant protease; Euphorbia