文章基本信息

标题：Development of In Vitro Conservation Medium of Carica pubescens Lenne & K. Koch through Nutrients Concentration Reduction and Osmoregulator Addition
其他标题：Pengembangan Medium Penyimpanan Carica pubescens Lenne & K. Koch Secara In Vitro dengan Reduksi Konsentrasi Nutrisi dan Penambahan Osmoregulator
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作者：Enni Suwarsi Rahayu ; Enni Suwarsi Rahayu ; Noor Aini Habibah 等
期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2015
卷号：7
期号：1
页码：22-28
DOI：10.15294/biosaintifika.v7i1.3538
语种：English
出版社：Universitas Negeri Semarang
摘要：Carica pubescens Lenne & K. Koch is a rare species that need to be conserved. The research aim was to develop a slow growth method of in vitro conservation medium through determining some effects of nutrition decreasing availability in the conservation medium on growth and survival of explants. Establishing epicotyls reached from in vitro seed germination was grown on diluted basic medium of 75% MS (Murashige and Skoog), 50% MS, 25% MS, while osmoregulator compound of mannitol and sorbitol was added to the full MS medium in several concentrations. The treatments were arranged in a completely randomized design with three replications. The epicotyls were grown at storage medium for 12 and 16 weeks, then their survival were evaluated at regeneration medium and rooting medium. The diluted basic medium and osmoregulator addition were evaluated for its influence in retarding the culture growth in terms of improved survival over the period of 16 weeks. Data analyzed by one way analysis of variance and Duncan’s multiple range test. The results showed that the decreasing of nutrition concentration suppressed the growth of the epicotyls until 16 weeks after conservation. Epicotyls taken from 16 weeks after conservation could grow on the regeneration medium. The best survival was shown by the 75% MS, 50% MS and supplementing of 20 g/l mannitol treatments. Based on these results, 50% MS medium is recommended for storage C. pubescens for 16 weeks with no sub-culture.
其他摘要：Carica pubescens Lenne & K. Koch (karika dieng) merupakan tanaman yang langka sehingga perlu dilestarikan. Penelitian ini bertujuan untuk memperoleh medium penyimpanan in vitro dengan teknik pertumbuhan minimal dengan mengamati pengaruh penurunan ketersed
关键词：in vitro conservation; slow growth; osmoregulator; C; pubescens