文章基本信息

标题：Optimization of 2,4-D Concentration, Ba, and Long Irradiation to Promote Regeneration Buds from Callus of Soybean
其他标题：Optimasi Konsentrasi 2,4-D, Ba, dan Lama Penyinaran untuk Memacu Regenerasi Tunas dari Kalus Kedelai
本地全文：下载
作者：Intan Kristanti ; Intan Kristanti ; Noor Aini Habibah 等
期刊名称：Biosaintifika: Journal of Biology & Biology Education
印刷版ISSN：2085-191X
电子版ISSN：2338-7610
出版年度：2014
卷号：5
期号：1
页码：50-57
DOI：10.15294/biosaintifika.v5i1.3923
语种：English
出版社：Universitas Negeri Semarang
摘要：Genetic trait improvement is a way to overcome the low quality soybean. The aim of this research is to determine the effect of 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Benzyl Adenine (BA) concentration and long irradiation and their interaction on bud regeneration from callus soybean, and to determine the interaction of the factors that most optimal in bud regeneration from callus soybean. 2,4-D and BA concentration each consisting of 4 levels (0 ppm; 3 ppm; 6 ppm; 9 ppm) and 2 levels long irradiation (24 hours and 0 hour). Analysis using a three -way ANAVA and Duncan test further. Parameters measured were the time appears bud, bud length, number of buds, and the percentage of callus forming buds. The result showed that BA concentration and long irradiation affects the regeneration of shoots from callus soybean, whereas the concentration of 2,4-D had no significant effect on bud regeneration. The most optimal concentration of BA is 3 ppm and optimal long irradiation is the condition 0 hours. BA concentration, 2,4-D concentration and long irradiation interaction effect on the regeneration of buds mainly on the number of buds produced. Treatment BA 3 ppm + 2,4-D 6 ppm + 24 hours is the optimal treatment in regenerating buds the number of callus that many. Based on the research results suggested to regenerate callus using ZPT BA and 2,4-D and long irradiation.
其他摘要：Alternatif untuk mengatasi kualitas kedelai yang rendah yaitu perbaikan sifat genetik. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan Benzyl Adenine (BA) serta lama penyinaran dan interaksinya ter
关键词：2;4-D; BA; irradiation; bud regeneration; Soybean callus