文章基本信息

标题：ISOLASI DAN PENAPISAN BAKTERI PENGHASIL ENZIM PROTEASE, SELULASE, DAN AMILASE DARI SEDIMEN DAN SALURAN PENCERNAAN TERIPANG HITAM Holothuria atra
其他标题：ISOLATION AND SCREENING OF PROTEASE, CELLULOSE, AND AMYLASE ENZYME SECRETION BACTERIA FROM SEDIMENT AND GUT OF SEA CUCUMBER Holothuria atra
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作者：Dien Arista Anggorowati ; Dien Arista Anggorowati ; Hendra Munandar 等
期刊名称：E-Journal Ilmu dan Teknologi Kelautan Tropis
印刷版ISSN：2087-9423
电子版ISSN：2085-6695
出版年度：2019
卷号：11
期号：2
页码：377-386
DOI：10.29244/jitkt.v11i2.21353
语种：English
出版社：Bogor Agricultural University
摘要：Sea cucumber Holothuria atra has potential value as raw material for pharmacy industry that has been cultivated in Research Development Division for Marine Bio Industry – Indonesian Institute of Sciences (LIPI) since 2016. Disease infections and decreased survival rate are one of the obstacles in in this type of sea cucumber cultivation activities especially during broodstock management. One effort to overcome this is the use of probiotic bacteria. This research aims to isolate and select bacteria that produce protease, cellulase, and amylase enzymes as candidates for probiotic bacteria in the sediment and digestive tract of sea cucumber H. atra. The growth of bacteria from sediments and digestive tract on selective media which added cellulose, starch, and casein substrates was carried out to see the potential of bacteria capable of producing cellulase, amylase, and protease enzymes through the hydrolysis process. The two bacterial isolates that had the highest cellulase and amylase enzyme activity were obtained, namely SSe9 isolates with Hydrolysis Capacity 7 mm index and SAm6 isolates with hydrolysis index Capacity 6.6 mm. These results indicate that the two bacterial isolates have the potential to be further developed into probiotic bacteria.
其他摘要：Teripang hitam Holothuria atra yang memiliki nilai potensial sebagai bahan baku pada industri farmasi telah dibudidayakan di Balai Bio Industri Laut – LIPI sejak tahun 2016. Infeksi penyakit dan penurunan sintasan hidup menjadi salah satu kendala dalam ke
关键词：Holothuria atra; enzyme activity; probiotic bacteria
其他关键词：Holothuria atra; aktivitas enzim; bakteri probiotik