文章基本信息

标题：PERUBAHAN KIMIA, MIKROBIOLOGIS DAN KARAKTERISTIK GEN HDC PENGKODE HISTIDIN DEKARBOKSILASE PADA IKAN TONGKOL ABU-ABU Thunnus tonggol SELAMA PENYIMPANAN SUHU DINGIN
其他标题：CHEMICAL, MICROBIOLOGY CHANGES AND DETECTION OF HDC GENE ON LONGTAIL TUNA Thunnus tonggol DURING CHILLING TEMPERATURE STORAGE
本地全文：下载
作者：Mala Nurilmala ; Mala Nurilmala ; Asadatun Abdullah 等
期刊名称：E-Journal Ilmu dan Teknologi Kelautan Tropis
印刷版ISSN：2087-9423
电子版ISSN：2085-6695
出版年度：2019
卷号：11
期号：2
页码：285-296
DOI：10.29244/jitkt.v11i2.23007
语种：English
出版社：Bogor Agricultural University
摘要：Histamine is a biogenic amine that appear during post moterm phase on the fish flesh that contain high content of histidine. The higher level of histamine can be reduced by good handling practice to maintain fish quality for example: using chilling temperature. This research aimed to determine chemical and microbiology changes from longtail tuna Thunnus tonggol and the time when hdc gene can be detected during chilling temperature storage 8±3°C. This research design was a completely randomized design (CRD) with parameters of differences in fish storage time (1,2,3,4,5,6,7 days) and ice ratio 1:1. The results showed that the tuna fish experienced quality deterioration for 7 days of storage. Organoleptic values and pH decreased during storage and on the seventh day the fish were in the rigormortis phase. TVB and TPC values increased during storage and on the sixth day storage has passed the safe limit for consumption. Histamine levels of this tuna on the seventh day were 1.96 ppm. HDc gene detection using the PCR method showed negative results in each treatment. The protein profile that was formed during storage displayed to separate because of the cathepsin activity.
其他摘要：Histamin merupakan senyawa amin biologis yang dapat terbentuk dari histidin bebas dalam daging ikan pada fase post rigor. Laju pertumbuhan histamin dapat diperlambat dengan cara menjaga mutu ikan menggunakan suhu dingin. Tujuan dari penelitian ini adalah
关键词：HDC gene; histamine; PCR; protein profile; TPC; TVB
其他关键词：gen HDC; histamine; PCR; profil protein; TPC; TVB