文章基本信息

标题：Genetic Relatedness Of Local Varieties Of Rice South Sumatra Based On Polymerase Chain Reaction – Random Amplified Polymorphic DNA (PCR-RAPD)
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作者：Laila Hanum ; Yuanita Windusari ; Muharni 等
期刊名称：Sriwijaya Journal of Environment
印刷版ISSN：2527-4961
电子版ISSN：2527-3809
出版年度：2017
卷号：2
期号：1
页码：19-23
DOI：10.22135/sje.2017.2.1.19-24
语种：English
出版社：Graduate Program Sriwijaya University
摘要：Diversity 22 local varieties of rice South Sumatra has the potential to be developed into rice varieties. The similarity (similarity) among 22 local varieties of rice South Sumatra became one of the obstacles for scientists to develop rice varieties. One solution to this problem is to determine the genetic relatedness between 22 rice local vaietas South Sumatra. Identification of genetic relatedness among 22 local varieties of rice South Sumatra can be carried out using PCR-RAPD. This study aims to determine the phylogenetic relationship among 22 local varieties of rice South Sumatra. This research about DNA isolation and visualitation has been carried out in August 2015 until December 2015 in Microbiology Laboratory and Laboratory of Genetics and Biotechnology,Department of Biology, Faculty of Mathematics and Natural Sciences,University of Sriwijaya and research about DNA quality and quantity test and PCR-RAPD has been done in Biotechnology Labotory Faculty of Mathematics and Natural Sciences, Sriwijaya University. Obtained 22 local varieties of rice accessions South Sumatra. Genetic relatedness identified using PCR-RAPD technique. 7 primer (OPA,OPA-9,OPA-10,OPA-13,OPA-16,OPA-19,OPB-8) was used for amplification. Local varieties of rice South Sumatra is divided into two major groups in association coefficient of 0.76,namely groups A and B. Group A consists of groups A1 and A2 in association coefficient of 0.78. In the large group B also consists of two groups: the B1 and B2 in association coefficient of 0.84. Local varieties of rice genetic relatedness South Sumatra are not influenced by geographical location..
其他摘要：Keanekaragaman 22 padi varietas lokal Sumatera Selatan memiliki potensi untuk dapat dikembangkan menjadi padi varietas unggul. Similaritas (kemiripan) diantara 22 padi varietas lokal Sumatera Selatan menjadi salah satu penghambat bagi para ilmuwan untuk m
关键词：Local Varieties Of Rice South Sumatra;Genetic Relatedness;PCR-RAPD..
其他关键词：Padi Varietas Lokal Sumatera Selatan;Hubungan Kekerabatan;PCR-RAPD.