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标题：Frequency of the Occurence of Methicilin Resistant Staphylococcus aureus Infections in Hyderabad,Pakistan
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作者：Nazir Ahmed Brohi ; Agha Asad Noor
期刊名称：Pakistan Journal of Analytical & Environmental Chemistry
印刷版ISSN：1996-918X
电子版ISSN：2221-5255
出版年度：2017
卷号：18
期号：1
页码：84-90
DOI：10.21743/pjaec/2017.06.08
语种：English
出版社：National Centre of Excellence in Analytical Chemsitry
摘要：Staphylococcus aureus is a potential pathogen of hospital and community related infections. It secretes toxins or the enzymes as virulence factor of mild to severe infections and show resistance to beta-lactam antibiotic including penicillin,methicillin,oxacillin and now vancomycin that could alarm of equal risk factors of Methicillin Resistant Staphylococcus aureus (MRSA) infections in the patients. The survey report of 381 patients of Hyderabad,Pakistan was collected from March 2013 to June 2014 in which 176 cases were reported for Staphylococcus aureus in both genders of different age groups of 3-15 y kids,16-45 y adults and 45-70 y olds,which showed 208 and 132 specimens Staphylococcus infection and 16 and 4 cases of MRSA infections in male and female patients,respectively whereas other 31 cases showed no infection. The laboratory diagnosis of the 200 samples from various hospitalized patients revealed the highest percentage of Methicillin Resistant Staphylococcus aureus MRSA in pus and post-operative wounds (17%) followed by skin swabs (10%),sputum (7%) and blood (0%). The observations revealed greater prevalence of MRSA infection in elderly age 16-45 years males than the females and other age groups. Antibiotic susceptibility test of 26 antibiotics revealed resistance (R-53%),sensitive (S-39) and variable (V-7%) sensitivity zones (mm). Amplification of mecA gene was done using PCR reaction that revealed mecA gene bands up to 150-200 base pairs by test resistant strains..
关键词：Staphylococcus aureus;Nosocomial infection;Antibiogram of MRSA;Methicillin resistance.