文章基本信息

标题：Development of Indirect Spectrophotometric Method for Quantification of Cephalexin in Pure Form and Commercial Formulation Using Complexation Reaction
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作者：Muhammad Naeem Khan ; Sundus Kalsoom ; Rahana Hussain 等
期刊名称：Pakistan Journal of Analytical & Environmental Chemistry
印刷版ISSN：1996-918X
电子版ISSN：2221-5255
出版年度：2016
卷号：17
期号：2
页码：118-123
DOI：10.21743/pjaec.v17i2.260
语种：English
出版社：National Centre of Excellence in Analytical Chemsitry
摘要：A simple,accurate and indirect spectrophotometric method was developed for the quantification of cephalexin in pure form and pharmaceutical products using complexation reaction. The developed method is based on the oxidation of the cephalexin with Fe3+ in acidic medium. Then 1,10- phenanthroline reacts with Fe2+ and a red colored complex was formed. The absorbance of the complex was measured at 510 nm by spectrophotometer. Different experimental parameters affecting the complexation reactions were studied and optimized. Beer’s law was obeyed in the concentration range 0.4 -10 µgmL-1 with a good correlation of 0.992. The limit of detection and limit of quantification were found to be 0.065 µgmL-1 and 0.218 µgmL-1,respectively. The method have good reproducibility with a relative standard deviation of 6.26 % (n = 6). The method was successfully applied for the determination of cephalexin in bulk powder and commercial formulation. Percent recoveries were found to range from 95.47 to 103.87 % for the pure form and 98.62 to 103.35 % for commercial formulations..
关键词：Cephalexin;Spectrophotometry;Pharmaceutical formulations;1;10-Phenonthroline.