文章基本信息

标题：Photoelectrochemical Sensors for the Rapid Detection of DNA Damage Induced by Some Nanoparticles
本地全文：下载
作者：M. Jamaluddin Ahmed ; Bin-Tian Zhang ; Liang-Hong Guo 等
期刊名称：Pakistan Journal of Analytical & Environmental Chemistry
印刷版ISSN：1996-918X
电子版ISSN：2221-5255
出版年度：2010
卷号：11
期号：1
页码：8-15
语种：English
出版社：National Centre of Excellence in Analytical Chemsitry
摘要：Photoelectrochemcal sensors were developed for the rapid detection of oxidative DNA damage induced by titanium dioxide and polystyrene nanoparticles. Each sensor is a multilayer film prepared on a tin oxide nanoparticle electrode using layer- by-layer self assembly and is composed of separate layer of a photoelectrochemical indicator,DNA. The organic compound and heavy metals represent genotoxic chemicals leading two major damaging mechanisms,DNA adduct formation and DNA oxidation. The DNA damage is detected by monitoring the change of photocurrent of the indicator. In one sensor configuration,a DNA intercalator,Ru(bpy)2 (dppz)2+ [bpy=2,2′ -bipyridine,dppz=dipyrido( 3,2-a: 2′ 3′-c) phenazine],was employed as the photoelectrochemical indicator. The damaged DNA on the sensor bound lesser Ru(bpy)2 (dppz)2+ than the intact DNA,resulting in a drop in photocurrent. In another configuration,ruthenium tris(bipyridine) was used as the indicator and was immobilized on the electrode underneath the DNA layer. After oxidative damage,the DNA bases became more accessible to photoelectrochemical oxidation than the intact DNA,producing a rise in photocurrent. Both sensors displayed substantial photocurrent change after incubation in titanium dioxide / polystyrene solution in a time – dependent manner. According to the data,damage of the DNA film was completed in 1h in titanium dioxide / polystyrene solution. In addition,the titanium dioxide induced much more sever damage than polysterene. The results were verified independently by gel electrophoresis and UV-Vis absorbance experiments. The photoelectrochemical reaction can be employed as a new and inexpensive screening tool for the rapid assessment of the genotoxicity of existing and new chemicals..
关键词：Photoelectrochemical sensors;DNA damage;Nanoparticles.