文章基本信息

标题：Application of the UHPLC-DIA-HRMS Method for Determination of Cheese Peptides
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作者：Georg Arju ; Anastassia Taivosalo ; Dmitri Pismennoi 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：8
页码：1-11
DOI：10.3390/foods9080979
出版社：MDPI Publishing
摘要：Until now, cheese peptidomics approaches have been criticised for their lower throughput. Namely, analytical gradients that are most commonly used for mass spectrometric detection are usually over 60 or even 120 min. We developed a cheese peptide mapping method using nano ultra-high-performance chromatography data-independent acquisition high-resolution mass spectrometry (nanoUHPLC-DIA-HRMS) with a chromatographic gradient of 40 min. The 40 min gradient did not show any sign of compromise in milk protein coverage compared to 60 and 120 min methods, providing the next step towards achieving higher-throughput analysis. Top 150 most abundant peptides passing selection criteria across all samples were cross-referenced with work from other publications and a good correlation between the results was found. To achieve even faster sample turnaround enhanced DIA methods should be considered for future peptidomics applications.
关键词：dairy product analysis; cheese peptidomics; cheesemaking; data-independent acquisition dairy product analysis ; cheese peptidomics ; cheesemaking ; data-independent acquisition