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标题：Purification and characterization of lysozyme in Persian sturgeon,Acipenser persicus (Borodin,1897) from the Southwest Caspian Sea
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作者：R Badan-Ara Marzdashti ; M.R Aghamaali ; A Varasteh 等
期刊名称：Caspian Journal of Environmental Sciences
印刷版ISSN：1735-3033
电子版ISSN：1735-3866
出版年度：2018
卷号：16
期号：4
页码：359-367
DOI：10.22124/CJES.2018.3204
语种：English
出版社：University of Guilan
摘要：Lysozyme (N-acetylmuramide glyconohydrolase,(EC 3.2.1.17)) is a unique enzyme which cleaves the β-1,4 linkages of N-acetylmuramic and N-acetylglucosamine of the peptidoglycan,which leads to the lysis of the bacterial cell wall.Lysozyme,as a self-defense enzyme,is produced in many organs of vertebrates.The present study describes purification and characterization of lysozyme from Acipenser persicus (Borodin,1897).After the extraction process,ion exchange chromatography was utilized to purify the enzyme.The SDS-PAGE analysis confirmed that the molecular weight was about 14 kDa.Moreover,some of the biochemical properties such as optimum temperature,pH and the effect of metal ions on the activity of purified enzyme were investigated.Based on the results the optimum activity and pH were obtained at 50 °C and 6.5 respectively.The purified lysozyme was active in the presence of different salts including NaCl (0–0.125 M),KCl (0.075–0.125 M),MgCl2,and CaCl2 (0.005 M).Kinetic parameters were also calculated.
关键词：Lysozyme;Acipenser persicus;Ion exchange chromatography;Metal ions;Optimum temperature;Catalytic efficiency