文章基本信息

标题：イノシシからの志賀毒素産生性大腸菌の分離と分離株の特徴
本地全文：下载
作者：木全恵子 ; 稲畑良 ; 佐賀由美子等
期刊名称：日本食品微生物学会雑誌
印刷版ISSN：1340-8267
电子版ISSN：1882-5982
出版年度：2020
卷号：37
期号：2
页码：61-68
DOI：10.5803/jsfm.37.61
出版社：Japanese Society of Food Microbiology
摘要：We isolated and investigated Shiga toxin (Stx)-producing Escherichia coli (STEC) from feces of captured wild boars in the Toyama Prefecture. For detection of STEC, which were not grown in STEC-selective medium, we cultured fecal samples in a non-STEC selective medium and screened the samples by stx -PCR. Out of fecal samples obtained from 76 wild boars, stx was detected in 12 samples (15.8%) and STEC strains were isolated from seven stx -positive fecal samples (9.2%). The STEC isolates harbored the following serotype and stx : Og8 : H19 ( stx2e ), Og148 : H10 ( stx2g ), Og105 : H7 ( stx2f ), and Og4 : H27 ( stx2g ). Except for Og105 : H7, the isolates were LEE (locus for enterocyte effacement)-negative STEC, which did not harbor the eae gene. Also, these isolates were not grown in STEC-selective medium. The wild boars harboring STEC were distributed in particular regions and STEC Og8 : H19 isolated from four wild boars captured from the same region were found to harbor the same DNA pattern by pulse-field gel electrophoresis (PFGE) analysis. Og148 : H10 and Og4 : H27 harbored virulence-associated genes, EAST-1 and heat-stable enterotoxin gene. The findings of our study indicate that wild boars are the carriers of LEE-negative STEC harboring virulence-associated genes that are different from those of the serotype O157 : H7, which was prevalent STEC in humans.
关键词：STEC;LEE-negative STEC;wild boar