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标题：High-Throughput Detection and Characterization of Antimicrobial Resistant Enterococcus sp. Isolates from GI Tracts of European Starlings Visiting Concentrated Animal Feeding Operations
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作者：Jennifer Anders ; Bledar Bisha
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：7
页码：1-13
DOI：10.3390/foods9070890
出版社：MDPI Publishing
摘要：Antimicrobial resistant enteric bacteria can easily contaminate the environment and other vehicles through the deposition of human and animal feces. In turn, humans can be exposed to these antimicrobial resistant (AMR) bacteria through contaminated food products and/or contaminated drinking water. As wildlife are firmly established as reservoirs of AMR bacteria and serve as potential vectors in the constant spread of AMR, limiting contact between wildlife and livestock and effective tracking of AMR bacteria can help minimize AMR dissemination to humans through contaminated food and water. Enterococcus spp., which are known opportunistic pathogens, constantly found in gastrointestinal tracts of mammalian and avian species, swiftly evolve and cultivate AMR genotypes and phenotypes, which they easily distribute to other bacteria, including several major bacterial pathogens. In this study, we evaluated the use of high throughput detection and characterization of enterococci from wildlife [European starlings (Sturnus vulgaris)] by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) following culture-based isolation. MALDI-TOF MS successfully identified 658 Enterococcus spp. isolates out of 718 presumptive isolates collected from gastrointestinal tracts of European starlings, which were captured near livestock operations in Colorado, Iowa, Kansas, Missouri, and Texas; antimicrobial susceptibility testing was then performed using 13 clinically significant antibiotics.
关键词：antimicrobial resistance; Enterococcus spp.; high throughput detection; matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS); culture-based isolation; susceptibility testing; wildlife; environment; concentrated animal feeding operations antimicrobial resistance ; Enterococcus spp. ; high throughput detection ; matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) ; culture-based isolation ; susceptibility testing ; wildlife ; environment ; concentrated animal feeding operations