文章基本信息

标题：Reducing the damage of quinoa saponins on human gastric mucosal cells by a heating process
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作者：Peng Xue ; Lei Zhao ; Yujie Wang 等
期刊名称：Food Science & Nutrition
电子版ISSN：2048-7177
出版年度：2020
卷号：8
期号：1
页码：500-510
DOI：10.1002/fsn3.1332
出版社：John Wiley & Sons, Ltd.
摘要：Different food processing methods will influence the structure and activity of compounds. In this work, molecular structure and different content crude saponins that were extracted from quinoa, treated with water soaking, water boiling, and water steaming were analyzed by HPLC. Flow cytometry was employed to investigate the effects of the main saponins on the GES‐1 cell line. HPLC/MS analysis revealed that water soaking induced an extensive conversion of polar saponin Qc (424.41 ± 21.11 mg/g) to the less polar compound Qf (247.04 ± 15.71 mg/g). After treatment with 100 μg of Qf instead of Qc for 24 hr, the percentage of dead cells increased from 20.1 ± 2.2% to 86.2 ± 4.8%. One major reason of this result is that less polar saponins could damage membrane integrity more easier than polar saponins. The results indicate that saponin toxicity is enhanced after degradation, so it is necessary to avoid degradation before use.
关键词：GES‐1 cell;heating;liquid chromatography;quinoa saponins