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标题：Complete genome sequence of “ Enterobacter lignolyticus ” SCF1
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作者：Kristen M. DeAngelis ; Patrik D’Haeseleer ; Dylan Chivian 等
期刊名称：Environmental Microbiome
印刷版ISSN：2524-6372
出版年度：2011
卷号：5
期号：1
页码：69-85
DOI：10.4056/sigs.2104875
摘要：In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated “Enterobacter lignolyticus” SCF1 on minimal media with alkali lignin as the sole source of carbon. This organism was isolated anaerobically from tropical forest soils collected from the Short Cloud Forest site in the El Yunque National Forest in Puerto Rico, USA, part of the Luquillo Long-Term Ecological Research Station. At this site, the soils experience strong fluctuations in redox potential and are net methane producers. Because of its ability to grow on lignin anaerobically, we sequenced the genome. The genome of “E. lignolyticus” SCF1 is 4.81 Mbp with no detected plasmids, and includes a relatively small arsenal of lignocellulolytic carbohydrate active enzymes. Lignin degradation was observed in culture, and the genome revealed two putative laccases, a putative peroxidase, and a complete 4-hydroxyphenylacetate degradation pathway encoded in a single gene cluster.
关键词：Anaerobic lignin degradation;tropical forest soil isolate;facultative anaerobe