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标题：Non contiguous-finished genome sequence and description of Microbacterium gorillae sp. nov.
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作者：Linda Hadjadj ; Jaishriram Rathored ; Mamadou Bhoye Keita 等
期刊名称：Environmental Microbiome
印刷版ISSN：2524-6372
出版年度：2016
卷号：11
期号：1
页码：1-9
DOI：10.1186/s40793-016-0152-z
摘要：Strain G3T (CSUR P207 = DSM 26203) was isolated from the fecal sample of a wild gorilla (Gorilla gorilla subsp gorilla) from Cameroon. It is a Gram-positive, facultative anaerobic short rod. This strain exhibits a 16S rRNA sequence similarity of 98.2 % with Microbacterium thalassium, the closest validly published Microbacterium species and member of the family Microbacteriaceae. Moreover, it shows a low MALDI-TOF-MS score (1.1 to 1.3) that does not allow any identification. Thus, it is likely that this strain represents a new species. Here we describe the phenotypic features of this organism, the complete genome sequence and annotation. The 3,692,770 bp long genome (one chromosome but no plasmid) contains 3,505 protein-coding and 61 RNA genes, including 4 rRNA genes. In addition, digital DNA-DNA hybridization values for the genome of the strain G3T against the closest Microbacterium genomes range between 19.7 to 20.5, once again confirming its new status as a new species. On the basis of these polyphasic data, consisting of phenotypic and genomic analyses, we propose the creation of Microbacterium gorillae sp. nov. that contains the strain G3T.
关键词：Microbacterium gorillae;Genome;Culturomics;Taxonomo-genomics;Gorilla stool sample