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标题：Draft genome sequence of Micromonospora sp. DSW705 and distribution of biosynthetic gene clusters for depsipeptides bearing 4-amino-2,4-pentadienoate in actinomycetes
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作者：Hisayuki Komaki ; Natsuko Ichikawa ; Akira Hosoyama 等
期刊名称：Environmental Microbiome
印刷版ISSN：2524-6372
出版年度：2016
卷号：11
期号：1
页码：1-10
DOI：10.1186/s40793-016-0206-2
摘要：Here, we report the draft genome sequence of Micromonospora sp. DSW705 (=NBRC 110037), a producer of antitumor cyclic depsipeptides rakicidins A and B, together with the features of this strain and generation, annotation, and analysis of the genome sequence. The 6.8 Mb genome of Micromonospora sp. DSW705 encodes 6,219 putative ORFs, of which 4,846 are assigned with COG categories. The genome harbors at least three type I polyketide synthase (PKS) gene clusters, one nonribosomal peptide synthetase (NRPS) gene clusters, and three hybrid PKS/NRPS gene clusters. A hybrid PKS/NRPS gene cluster encoded in scaffold 2 is responsible for rakicidin synthesis. DNA database search indicated that the biosynthetic gene clusters for depsipeptides bearing 4-amino-2,4-pentadienoate are widely present in taxonomically diverse actinomycetes.
关键词：Actinomycete;BE-43547;Micromonospora;Nonribosomal peptide synthetase;Polyketide synthase;Rakicidin;Taxonomy;Vinylamycin