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标题：Complete genome sequence of lytic bacteriophage RG-2014 that infects the multidrug resistant bacterium Delftia tsuruhatensis ARB-1
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作者：Ananda Shankar Bhattacharjee ; Amir Mohaghegh Motlagh ; Eddie B. Gilcrease 等
期刊名称：Environmental Microbiome
印刷版ISSN：2524-6372
出版年度：2017
卷号：12
期号：1
页码：1-14
DOI：10.1186/s40793-017-0290-y
摘要：A lytic bacteriophage RG-2014 infecting a biofilm forming multidrug resistant bacterium Delftia tsuruhatensis strain ARB-1 as its host was isolated from a full-scale municipal wastewater treatment plant. Lytic phage RG-2014 was isolated for developing phage based therapeutic approaches against Delftia tsuruhatensis strain ARB-1. The strain ARB-1 belongs to the Comamonadaceae family of the Betaproteobacteria class. RG-2014 was characterized for its type, burst size, latent and eclipse time periods of 150 ± 9 PFU/cell, 10-min, <5-min, respectively. The phage was found to be a dsDNA virus belonging to the Podoviridae family. It has an isometric icosahedrally shaped capsid with a diameter of 85 nm. The complete genome of the isolated phage was sequenced and determined to be 73.8 kbp in length with a G + C content of 59.9%. Significant similarities in gene homology and order were observed between Delftia phage RG-2014 and the E. coli phage N4 indicating that it is a member of the N4-like phage group.
关键词：Bacteriophage;Delftia tsuruhatensis;Multidrug resistant;Biofouling;Biofilm;Genome;Podoviridae