文章基本信息

标题：A performance evaluation of targeted eDNA and eDNA metabarcoding analyses for freshwater fishes
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作者：Jonas Bylemans ; Dianne M. Gleeson ; Richard P. Duncan 等
期刊名称：Environmental DNA
电子版ISSN：2637-4943
出版年度：2019
卷号：1
期号：4
页码：402-414
DOI：10.1002/edn3.41
出版社：Wiley
摘要：Background The use of environmental DNA analysis has revolutionized biodiversity monitoring. Initially, eDNA monitoring surveys in aquatic environments utilized a targeted approach, but there has been a steady shift toward whole community assessments (eDNA metabarcoding). Both approaches can increase the detection sensitivity for rare and elusive species, compared to more conventional methods. However, it is important to understand the benefits and limitations of targeted and whole community eDNA monitoring to tailor surveys to research questions and management objectives.
关键词：eDNA metabarcoding;environmental DNA;fishes;real‐time PCR