文章基本信息

标题：Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA
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作者：Kimiko Uchii ; Hideyuki Doi ; Teruyuki Okahashi 等
期刊名称：Environmental DNA
电子版ISSN：2637-4943
出版年度：2019
卷号：1
期号：4
页码：359-367
DOI：10.1002/edn3.37
出版社：Wiley
摘要：Background Real‐time PCR has been widely employed in environmental DNA (eDNA) surveys to detect and quantify target species. The biggest obstacle in real‐time PCR based eDNA assays is the inhibition of PCR amplification that results in false‐negative detection and uncertainty of quantification.
关键词：fulvic acid;humic acid;PCR inhibition;real‐time PCR;tannic acid