文章基本信息

标题：Cross reaction and forensic comparison of blood testing done by private and public sector laboratories
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作者：Christian Stadler ; Claudemir Rodrigues Dias Filho ; Maria Gabriela Roca 等
期刊名称：Revista Brasileira de Criminalística
印刷版ISSN：2237-3691
电子版ISSN：2237-9223
出版年度：2017
卷号：6
期号：2
页码：31-37
DOI：10.15260/rbc.v6i2.169
出版社：Associação Brasileira de Criminalística
摘要：Blood evidence consists of one of the most important forensic pieces. However, it is not always easy to identify a stain as blood. The best and fastest way of doing so, is by using rapid screening derived from immunochromatographic tests to spot hemoglobin. However, cross reaction between species might be an issue since hemoglobin of other animals could be present, especially considering crimes against fauna, outdoor crime scenes with an easy access for animals, and human and domestic animal trauma cases. According to the particular structure in each country there are private and/or public laboratories working in this field. Methods and resources from private and public sectors are different, and procedures also differ from country to country. The main objective of our work was to validate the presence of human blood using interspecific samples and the criteria defined by each lab using rapid blood detection test kits from the public sector related to forensic analysis in Brazil and from the private sector in Germany. As a secondary goal, we have evaluated sensibility, the Hook Effect and pH effect in some commercially available kits. Our results showed that the tests carried out are suitable for the detection of human blood in forensic samples using methods commonly employed in the public and private sectors.
其他摘要：Blood evidence consists of one of the most important forensic pieces. However, it is not always easy to identify a stain as blood. The best and fastest way of doing so, is by using rapid screening derived from immunochromatographic tests to spot hemoglobin. However, cross reaction between species might be an issue since hemoglobin of other animals could be present, especially considering crimes against fauna, outdoor crime scenes with an easy access for animals, and human and domestic animal trauma cases. According to the particular structure in each country there are private and/or public laboratories working in this field. Methods and resources from private and public sectors are different, and procedures also differ from country to country. The main objective of our work was to validate the presence of human blood using interspecific samples and the criteria defined by each lab using rapid blood detection test kits from the public sector related to forensic analysis in Brazil and from the private sector in Germany. As a secondary goal, we have evaluated sensibility, the Hook Effect and pH effect in some commercially available kits. Our results showed that the tests carried out are suitable for the detection of human blood in forensic samples using methods commonly employed in the public and private sectors.
关键词：Identificação de sangue humano; Hemoglobina; Efeito Hook; Testes imunocromatográficos; Testes rápidos.
其他关键词：Human blood identification;Hemoglobin;High Dose Hook effect;Immunochromatographic tests;Rapid screening tests