文章基本信息

标题：Resazurin Assay Data for Mycobacterium tuberculosis Supporting a Model of the Growth Accelerated by a Stochastic Non-Homogeneity
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作者：Eugene B. Postnikov ; Andrey A. Khalin , Anastasia I. Lavrova ; Olga A. Manicheva 等
期刊名称：Data
印刷版ISSN：2306-5729
出版年度：2019
卷号：4
期号：1
页码：36-43
DOI：10.3390/data4010036
出版社：MDPI Publishing
摘要：Tuberculosis is one of the most widespread worldwide diseases heavily affecting society. Among popular modern laboratory tests for mycobacterial growth, the resazurin assay has certain advantages due to its effectiveness and relatively low cost. However, the high heterogeneity of the mycobacterial population affects the average growth rate. This fact must be taken into account in a quantitative interpretation of these tests’ output—fluorescence growth curves—related to the population growth of viable mycobacteria. Here, we report the spectrophotometric data obtained via the resazurin assay for the standard reference strain of Mycobacterium tuberculosis H37Rv for different initial dilutions and generation numbers of the culture, as well as their primary processing from the point of view of the stochastic multiplicative growth model. The obtained data, which indicate an accelerated (instead of linear) growth of the population density logarithm between the end of the lag phase and the saturation, provide evidence of the importance of the growth rates’ stochasticity. An analysis of the curve fits resulted in an estimation of the first two moments of the growth rates’ probability distributions, showing its relevance to vital processes for mycobacterial culture.
关键词：Mycobacterium tuberculosis ; stochastic growth rates; population growth models; accelerated growth Mycobacterium tuberculosis ; stochastic growth rates ; population growth models ; accelerated growth