文章基本信息

标题：Metastatic and non-metastatic sentinel inguinofemoral lymph nodes in vulvar cancer show an increased lymphangiogenesis
本地全文：下载
作者：Magda Piechowicz ; Marcin Mikos ; Tomasz Banas 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2020
卷号：27
期号：1
页码：123-128
DOI：10.26444/aaem/105925
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction and objective. Lymph node involvement is a strong predictor of disease recurrence and patient survival invulvar cancer. The aim of the study was to evaluate the feasibility of sentinel lymph node (SLN) screening, the incidence ofskip metastases, and lymph node lymphangiogenesis.Materials and method. Fifty-five patients participated in this prospective, single centre study. A double SLN screeningmethod was employed using radiocolloid (technetium-99 sulfur colloid) and 1.0% Isosulfan Blue. Immunohistochemistry,using a mouse monoclonal antibody against D2–40, was used to evaluate lymphatic vessel density (LVD). All calculationswere performed using STATISTICA software v. 10 (StatSoft, USA, 2011); p < 0.05 was considered significant.Results. Using both methods of SLN detection, 100% accuracy was achieved, and skip metastases were diagnosed in onlyone woman (1.82%). Peri-tumour median LVD was significantly increased compared with matched intra-tumour samples(p < 0.001), while median LVD was significantly lower in negative, compared with positive SLN, regardless of whether matchednon-SLN were negative (p < 0.001) or positive (p = 0.005). Metastatic SLN exhibited significantly higher median LVD comparedwith matched negative non-SLN (p = 0.015), while no significant difference in median LVD was detected between positiveSLN and matched positive non-SLN. However, negative SLN had a significantly higher median LVD compared with matchednegative non-SLN (p = 0.012).Conclusions. SLN detection is a safe and feasible procedure in vulvar cancer. In patients without nodular involvement, SLN,compared with non-SLN, exhibited significantly higher median LVD, which may be an indication of its preparation to hostmetastases, and thus requires further investigation.
关键词：anti;D2–40 antibody; lymphatic vessel density; immunohistochemistry; sentinel lymph node; vulvar cancer