文章基本信息

标题：Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests
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作者：Agung Sosiawan ; Dadik Raharjo ; Indah Nuraini 等
期刊名称：Egyptian Journal of Forensic Sciences
电子版ISSN：2090-5939
出版年度：2018
卷号：8
期号：1
页码：1-5
DOI：10.1186/s41935-018-0047-9
摘要：IntroductionA prenatal paternity test is one widely-used method of determining the paternity of an unborn child. Such tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. In the present day, a prenatal paternity test using circulating cell-free fetal Deoxyribonucleic Acid (DNA) is one alternative method due to it being non-invasive and safe for both mother and foetus.AimThe aim of this study is to detect Short Tandem Repeat (STR) at 5 Loci (vWA, TH01, D13S317, D18S51, and D21S11) and amelogenin genes in circulating cell-free fetal DNA in paternity tests.Materials and methods 200 bp and < 200 bp was also conducted to establish the sensitivity of the test.ResultsThere was a significant difference between maternal blood DNA and circulating cell-free fetal DNA ( p = 0.000 D13S317; p = 0.000 D21S11; p = 0.000 D18S51; p = 0.000 vWA; p = 0.000 TH01; and p = 0.000 amelogenin genes). The locus 200 bp.ConclusionCirculating free fetal DNA can be used as an alternative sample for prenatal paternity tests because of its similarity with maternal DNA.
关键词：Early detection; Circulating free fetal DNA; Non-invasive technique; Prenatal paternity test