文章基本信息

标题：Development of a Turn-On Fluorescent Traceable Linker Employing N-Sulfanylethylcoumarinyl Amide for the Enrichment and Visualization of Target Proteins
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作者：Takuya Morisaki ; Akira Shigenaga ; Akira Otaka 等
期刊名称：Chemical and Pharmaceutical Bulletin
印刷版ISSN：0009-2363
电子版ISSN：1347-5223
出版年度：2020
卷号：68
期号：3
页码：216-219
DOI：10.1248/cpb.c19-00726
出版社：The Pharmaceutical Society of Japan
摘要：A turn-on fluorescent traceable linker based on N -sulfanylethylcoumarinyl amide (SECmide) has been developed as an advanced cleavable linker. It was successfully employed for the enrichment and selective visualization of a target protein in cell lysate. The results demonstrated that the SECmide-based traceable linker is potentially applicable to the identification of low molecular weight target proteins, a factor which has been problematic for a previously developed N -sulfanylethylanilide-based traceable linker..
关键词：cleavable linker;N;sulfanylethylanilide;N;sulfanylethylcoumarinyl amide;target protein;traceable linker;turn;on fluorescent