文章基本信息

标题：Equol Inhibits Mushroom Tyrosinase in Vitro through Tight Binding
本地全文：下载
作者：Toshio Niwa ; Haruna Akiyama ; Madoka Echikawa 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2020
卷号：43
期号：3
页码：550-553
DOI：10.1248/bpb.b19-00756
出版社：The Pharmaceutical Society of Japan
摘要：Equol, an intestinal metabolite of daidzein, inhibited more potently mushroom tyrosinase in vitro than other inhibitors, genistein and kojic acid. We investigated the mechanism underlying tyrosinase inhibition by equol. Treating racemic equol with tyrosinase produced 3ʹ-hydroxyequol. Because the optical activity of the product showed <25% enantiomeric excess, the reaction was not highly stereospecific. Using enzyme-linked immunosorbent assays with an anti-equol monoclonal antibody, we observed that equol bound to pre-coated tyrosinase in a dose-dependent manner. Our results suggested the formation of a stable equol–tyrosinase complex..
关键词：equol;tyrosinase;genistein;enzyme;linked immunosorbent assay (ELISA)