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标题：Blood flow restriction in human skeletal muscle during rest periods after high-load resistance training down-regulates miR 206 and induces Pax7
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作者：Ferenc Torma ; Zoltan Gombos ; Marcell Fridvalszki 等
期刊名称：Journal of Sport and Health Science
印刷版ISSN：2095-2546
出版年度：2019
页码：1-8
DOI：10.1016/j.jshs.2019.08.004
出版社：Elsevier
摘要：Purpose Blood flow restriction (BFR) with low-intensity resistance training has been shown to result in hypertrophy of skeletal muscle. In this study, we tested the hypothesis that BFR during the rest periods between acute, high-intensity resistance exercise sessions (70% of 1 repetition maximum, 7 sets with 10 repetitions) enhances the effects of the resistance training. Methods A total of 7 healthy young men performed squats, and between sets BFR was carried out on 1 leg while the other leg served as a control. Because BFR was applied during rest periods, even severe occlusion pressure (approximately 230 mm Hg), which almost completely blocked blood flow, was well-tolerated by the participants. Five muscle-specific microRNAs were measured from the biopsy samples, which were taken 2 h after the acute training. Results Doppler data showed that the pattern of blood flow recovery changed significantly between the first and last BFR. MicroRNA-206 levels significantly decreased in the BFR leg compared to the control. The mRNA levels of RAC-β serine/threonine-protein kinase v22, nuclear respiratory factor 1, vascular endothelial growth factor, lupus Ku autoantigen protein p70 genes ( p < 0.05), and paired box 7 ( p < 0.01) increased in the BFR leg. The protein levels of paired box 7, nuclear respiratory factor 1, and peroxisome proliferator-activated receptor γ coactivator 1α did not differ between the BFR leg and the control leg. Conclusion Data revealed that BFR, during the rest periods of high-load resistance training, could lead to mRNA elevation of those proteins that regulate angiogenesis, mitochondrial biogenesis, and muscle hypertrophy and repair. However, BFR also can cause DNA damage, judging from the increase in mRNA levels of lupus Ku autoantigen protein p70.
关键词：Blood flow restriction ; High-intensity resistance training ; microRNA ; Satellite cells