文章基本信息

标题：GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A
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作者：Gebhard Schratter ; Susanne Scheruebel ; Sonja Langthaler 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2019
卷号：9
期号：1
页码：1-18
DOI：10.1038/s41598-019-55683-w
出版社：Springer Nature
摘要：Excessive expression of subunit 1 of GIRK1 in ER + breast tumors is associated with reduced survival times and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells were engineered to overexpress GIRK1 neoplasia associated vital parameters and resting potentials were measured and compared to controls. The presence of GIRK1 resulted in resting potentials negative to the controls. Upon GIRK1 overexpression, several cellular pathways were regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10A GIRK1 with the control (MCF10A eGFP ). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, but to a lesser extent. Thus, GIRK1 regulated cellular pathways in mammary epithelial cells are likely to contribute to the development and progression of breast cancer.