文章基本信息

标题：Bluephage, a method for efficient detection of somatic coliphages in one hundred milliliter water samples
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作者：Javier Méndez ; Daniel Toribio-Avedillo ; Raquel Mangas-Casas 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2020
卷号：10
期号：1
页码：1-5
DOI：10.1038/s41598-020-60071-w
出版社：Springer Nature
摘要：Emerging water quality guidelines and regulations require the absence of somatic coliphages in 100 mL of water, yet the efficiency of standardized methods to test this volume of sample is questionable. A recently described procedure, Bluephage, using a modified E. coli host strain, overcomes some of the methodological limitations of standardized methods. In a maximum of 6.5 hours (2.5 hours for pre-growing the host strain and 4 hours for the presence/absence test), Bluephage allows the direct detection of one plaque-forming unit (PFU) in a 100 mL water sample. The test shows high levels of specificity for somatic coliphages and comparable accuracy with standardized methods.