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标题：Studies on the Prevalence of E. coli in Chicken Carcasses in Abattoirs and its Antibiotic Sensitivity -
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作者：Mahmoud Abd Elzaher ; Ebeed A. Saleh ; Reem Abd Elhamied 等
期刊名称：Alexandria Journal of Veterinary Sciences
印刷版ISSN：1110-2047
电子版ISSN：1110-2047
出版年度：2018
卷号：58
期号：1
页码：132-138
DOI：10.5455/ajvs.285297
出版社：International Medical Journal Management and Indexing System
摘要：This study aimed to estimate Butchers hands and articles as a source ofE. coli contamination in poultry meat and slaughterhouses andto estimate the effect of random antimicrobials usage on the antimicrobial susceptibility of the isolated E. coli. A total of 192 samples were collected from four poultry abattoirs in El-Beheira governorate. Samples were collected from butchers' hands, articles, carcass internal and external surfaces. E. coli was isolated and identified by culture, biochemical analysis and PCR. Further the isolates were studied for their antimicrobial susceptibility against 11 commonly used antibiotics in poultry farms. E. coliwas detected in hands (87.5%), articles(81.25%), internal(80.35%) and external (78.57%) surfaces of the carcasses. The highest antimicrobial sensitivity of the isolates were detected to Tobramycin (100%), Cefotaxime (50-75%) and Chloramphenicol (59-66.7%). While, the highest resistance was detected to Penicillin, Sulfamethoxazole and Trimethoprin, (100%), Amoxicillin (93- 100%), Cephradine and Doxycycline. Further, multiple antimicrobial resistance was detected. By PCR, 33.3% (2/6) of the samples from the internal surface of the carcasses were positive for the intimin; eaeA gene. Thus, contamination of chicken carcasses in the abattoirs with pathogenic E. coli that resists different antimicrobials poses a challenge for human food and thus human health. The use of antimicrobials as well asapplying hygienic proceduresin abattoirs are clearly a necessity for the production of healthy food.
关键词：E. coli; abattoir; chicken carcasses; antimicrobial sensitivity