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标题：Growth Response of Lactobacillus rhamnosus Chloramphenicol-Resistant Strain ATCC 27773 to Pteroyl-Mono- and -Di-Glutamates
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作者：Kyohei KOSEKI ; Naho OKAMOTO ; Tomohiro BITO 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2019
卷号：65
期号：6
页码：545-549
DOI：10.3177/jnsv.65.545
出版社：Center for Academic Publications Japan
摘要：This study investigated the effect of various concentrations of pteroyl-mono-γ-glutamate (PteGlu 1 ) and pteroyl-di-γ-glutamate (PteGlu 2 ) on the growth of Lactobacillus rhamnosus strains ATCC 7469 (wild-type strain) and ATCC 27773 (chloramphenicol-resistant strain) used for folate microbiological assays. At concentrations of 0.025-0.20 nmol/L, the growth of the chloramphenicol-resistant strain was stimulated to a greater extent by PteGlu 1 than by PteGlu 2 , but the wild-type strain did not show such phenomena. L. rhamnosus ATCC 27773 bioassays were used to determine the total folate content of various foods treated with a chicken pancreas folate conjugase. This showed a significantly lower value when PteGlu 1 was used as a calibrator than with PteGlu 2 . These results indicated that PteGlu 2 should be the standard folate compound when chicken pancreas folate conjugase is used in preparing samples for L. rhamnosus ATCC 27773 bioassay..
关键词：folate conjugase;folate γ;glutamyl hydrolase;microbiological folate assay;pteroyl;poly;γ;glutamates;total folate determination