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标题：Advancement in Vaccination of Broiler Chickens with Genotype-Matched Vaccines to Currently Epidemic Newcastle Disease Virus Genotype VII in Egypt.
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作者：Amer SAM ; Ali MA ; Kandeil AM 等
期刊名称：Journal of World's Poultry Research
电子版ISSN：2322-455X
出版年度：2019
卷号：9
期号：3
页码：1-7
出版社：Scienceline Publication
摘要：Newcastle disease virus (NDV) outbreaks still occur frequently in Egypt in spite of the heavy implementation of classic NDV vaccines for a long time ago, where NDV genotype VII has become the dominant genotype in Egypt from 2012 until now. Many previous studies have recommended using genotype-matched NDV vaccines against the epidemic virus for providing better protection and minimizing virus shedding. Therefore, the present study evaluated the efficacy of two available live NDV vaccines in Cobb 500 broilers. The group A and B (20 birds each) were vaccinated with live attenuated NDV vaccines genotype VII and II, respectively with double doses at 5 and 19 days of age. Also, group C consisting of 20 unvaccinated birds was studied as a control group. The efficacy of live vaccines was determined using virus challenge test. Hence, all groups were challenged with velogenic NDV genotype VIId at a dose equivalent to 10 6.0 50 percent Embryo Infective Dose (EID50) via the intramuscular route at 28 daysold. Serum antibodies level was assessed by hemagglutination inhibition test. Moreover, virus shedding was measured by EID50. The obtained results indicated that vaccinated birds had similar haemagglutination titers with no significant difference prior challenge. Meanwhile, group A showed significant protection against mortality, as well as a significant reduction in virus shedding 7 days post-challenge compared to Group B. We concluded that live recombinant-genotype VII vaccine homologous to challenge virus could improve the protective efficiency in chicken against NDV compared to live classic genotype II vaccine. It is suggested that the implementation of genotypematched NDV vaccines confer better protection in commercial broilers vaccination programs.
关键词：Broilers; Genotype;matched vaccine; Genotype VII; Newcastle disease virus