文章基本信息

标题：Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone
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作者：Kai-Hsi Liu , , Danny O’Hare , James L. Thomas , Han-Zhang Guo , Chien-Hsin Yang ; Mei-Hwa Lee
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2020
卷号：10
期号：3
DOI：10.3390/bios10030016
出版社：MDPI Publishing
摘要：Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings and functional groups, which can undergo π－π and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted electronically conductive polymers (TIECPs) on sensing electrodes. The linear sensing range for testosterone was from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000-fold to measure testosterone concentration using the above TIECP sensors; results were compared with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33 ± 0.09 to 9.13 ± 1.33 ng/mL. The mean accuracy of the TIECP-coated sensors was 90.3 ± 7.0%.
关键词：testosterone; molecular imprinting; electronically conductive polymer; electrochemical sensing; urine testosterone ; molecular imprinting ; electronically conductive polymer ; electrochemical sensing ; urine