文章基本信息

标题：All-optical targeted drug delivery and real-time detection of a single cancer cell
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作者：Xiaole Liu ; Jie Yuan ; Dong Wu 等
期刊名称：Nanophotonics
印刷版ISSN：2192-8606
电子版ISSN：2192-8614
出版年度：2020
卷号：9
期号：3
页码：611-622
DOI：10.1515/nanoph-2019-0318
出版社：Walter de Gruyter GmbH
摘要：Targeted drug delivery and real-time detection both play an important role for studying the specificity of a single cancer cell and the development of anticancer drugs. However, a method that simultaneously enables safe and efficient targeted drug delivery and noninvasive, free-label cell detection is highly desirable but challenging. Here, we report an all-optical method that combines fiber optical tweezers with laser Raman microspectroscopy, which can achieve targeted drug delivery to a single cancer cell using optical manipulation in vitro quickly and accurately by a tapered fiber probe, and simultaneously record the corresponding active characteristics of the targeted cancer cell under the contact of delivered drug through a Raman spectrometer. Using the method, drug delivery and release can be flexibly controlled by turning on/off the trapping laser beam propagating in the fiber, which can avoid the complex systems and is highly autonomous and controllable. Moreover, the detection of cell activity does not require any dye calibration and processing, and it is noninvasive. In addition, for a single suspension cell, optical trapping of the cell using another fiber tip can overcome the low efficiency of targeted drug delivery and the poor stability of the Raman spectrum caused by Brownian motion of the cell. This all-optical method provides a promising approach to conduct pharmacologic studies with the reaction of cancer cell and drugs at the level of a single cell.
关键词：optical tweezers ; Raman detection ; drug delivery