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标题：Imaging breast cancer using hyperpolarized carbon-13 MRI
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作者：Ferdia A. Gallagher ; Ramona Woitek ; Mary A. McLean 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2020
卷号：117
期号：4
页码：2092-2098
DOI：10.1073/pnas.1913841117
出版社：The National Academy of Sciences of the United States of America
摘要：Our purpose is to investigate the feasibility of imaging tumor metabolism in breast cancer patients using ¹³C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized ¹³C label exchange between injected [1-¹³C]pyruvate and the endogenous tumor lactate pool. Treatment-naïve breast cancer patients were recruited: four triple-negative grade 3 cancers; two invasive ductal carcinomas that were estrogen and progesterone receptor-positive (ER/PR+) and HER2/neu-negative (HER2−), one grade 2 and one grade 3; and one grade 2 ER/PR+ HER2− invasive lobular carcinoma (ILC). Dynamic ¹³C MRSI was performed following injection of hyperpolarized [1-¹³C]pyruvate. Expression of lactate dehydrogenase A (LDHA), which catalyzes ¹³C label exchange between pyruvate and lactate, hypoxia-inducible factor-1 (HIF1α), and the monocarboxylate transporters MCT1 and MCT4 were quantified using immunohistochemistry and RNA sequencing. We have demonstrated the feasibility and safety of hyperpolarized ¹³C MRI in early breast cancer. Both intertumoral and intratumoral heterogeneity of the hyperpolarized pyruvate and lactate signals were observed. The lactate-to-pyruvate signal ratio (LAC/PYR) ranged from 0.021 to 0.473 across the tumor subtypes (mean ± SD: 0.145 ± 0.164), and a lactate signal was observed in all of the grade 3 tumors. The LAC/PYR was significantly correlated with tumor volume ( R = 0.903, P = 0.005) and MCT 1 ( R = 0.85, P = 0.032) and HIF1α expression ( R = 0.83, P = 0.043). Imaging of hyperpolarized [1-¹³C]pyruvate metabolism in breast cancer is feasible and demonstrated significant intertumoral and intratumoral metabolic heterogeneity, where lactate labeling correlated with MCT1 expression and hypoxia.
关键词：metabolic imaging ; magnetic resonance imaging ; cancer metabolism ; breast cancer