文章基本信息

标题：Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography
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作者：Lavinia Gambelli ; Benjamin H. Meyer ; Mathew McLaren 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2019
卷号：116
期号：50
页码：25278-25286
DOI：10.1073/pnas.1911262116
出版社：The National Academy of Sciences of the United States of America
摘要：Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable protective barrier around the cell, and cell–cell interaction, as well as surface adhesion. Despite the central importance of S-layers for archaeal life, their 3-dimensional (3D) architecture is still poorly understood. Here we present detailed 3D electron cryomicroscopy maps of archaeal S-layers from 3 different Sulfolobus strains. We were able to pinpoint the positions and determine the structure of the 2 subunits SlaA and SlaB. We also present a model describing the assembly of the mature S-layer.
关键词：S-layers ; electron cryotomography ; Sulfolobus ; archaea ; subtomogram averaging