Healthcare-Associated Infections (HAIs) represent a frequent complication for hospitalized patients and more rarely for workers. In recent years, substantial scientific evidence has been reached regarding the role played by the inanimate surfaces, especially those touched in patient-care areas, in the transmission of nosocomial pathogens. Therefore, it is essential to find new collective protective measures to minimize microbial contamination in healthcare facilities, thereby preventing the spread of multi-drug resistant bacteria. We present an overview of the major nano-enabled AntiMicrobial Coatings (AMCs) which may be used as collective protective measures in healthcare setting, discussing also some aspects related to their effectiveness and safety. AMCs may be classified within three groups on base of their mechanism of action: surfaces releasing active compound, contact-killing surfaces and anti-adhesive surfaces. To date, little information is available on the effectiveness of AMCs to reduce the risk of HAIs since the most of studies do not reach conclusive results on their beneficial effects. Moreover, the lack of standard protocols for assessing antimicrobial efficacy and poor data about the interaction between AMCs and disinfectants prevent their placing on the market. Further studies are needed for assessing risks and benefits of AMCs as collective protective measures in healthcare setting.