文章基本信息

标题：Covalent Immobilization of β -Galactosidase Enzyme onto Modified Alginate Gel Beads
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作者：M.E. Hassan ; M.E. Hassan ; A.G. Ibrahim 等
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2018
卷号：9
期号：9
页码：2483-2492
出版社：University of Mohammed Premier Oujda
摘要：Alginate / H0 (acrylamide-co-acrylic acid) gel beads was generated and modified bysequential soaking with polyethyleneimine (PEI) and glutaraldehyde (GA) as a functionalcarrier suitable for enzymes immobilization. The modification was carried out by usingresponse surface methodology. Maximum immobilization yield (78.2%) of β-galactosidase was obtained by soaking the gel beads with 3.5% of (PEI) for 5hrs. followedby soaking the treated gel beads with 4 % of (GA) for 6hrs. Analysis of variance(ANOVA) showed a high coefficient of determination value (R2) of 0.90, ensuring asatisfactory adjustment of the quadratic model with the experimental data. Thermogravimetric analysis (TGA) of gel beads during different improvement steps showed aremarkable increase in their thermal stability from 190, 200 and 210 °C for Alginate-H0,Alginate-H0 / PEI and Alginate-H0 / PEI / GA respectively. The reusability test provedthe durability of the modified Alginate / H0 (acrylamide-co-acrylic acid) gel beads for 7cycles with retention of 100% of the immobilized enzyme activity losing only 10% of itsactivity after 17 cycles. To be more convenient for industrial uses , considerable stabilityand reusability of bound enzyme maybe advantageous for its industrial application.
关键词：Alginate;Acrylamide;Immobilization;Modification;Response surface;methodology.