文章基本信息

标题：Production of Pathogenesis-Related proteins during the induction of resistance to Phytophthora capsici in pepper plants treated with Burkholderia cepacia and Trichoderma harzianum in combination compatible
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作者：M. Ezziyyani ; M. Ezziyyani ; A. Hamdache 等
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2017
卷号：8
期号：s
页码：4785-4795
出版社：University of Mohammed Premier Oujda
摘要：We evaluate the defensive reaction of pepper plants after inoculating the stems withPhytophthora capsici using a decapitation method and treating the roots with theantagonists Burkholderia cepacia and Trichoderma harzianum jointly. Infection of thestems with the pathogen produces a hypersensitivity reaction but necrosis is sloweddown in plants treated with the two antagonists, as a result of induced resistance. Theaddition of the antagonists to plants inoculated with P. capsici produces a defensivereaction involving the production of proteins showing β-1,3-glucanase activity. Theincrease in activity affects both constitutive enzymes and those synthesised “de novo”,although the increase in activity detected in control plants is due to the effect of thewound rather than to the treatment since it does not increase with time. Treatment withthe antagonists induces a systemic reaction in the roots and stems, which is resolved bythe production of PR proteins with β-1,3-glucanase activity, even though the stems arenot affected by the antagonist or by the pathogen. These PR-glucanases are basic (pI 9.0and pI 7.8) and PR-glucanase acid (pI 4.5) are detected in stems and roots treated withantagonistic’s 3 and 9 days after inoculation, common in stems and roots and in thedifferent treatments of time but that differ in their concentration. Increased β-1,3-glucanase activity is also observed in stems and roots and the production of a PR proteinis observed both at 9 days. This isoenzyme is acidic (RF 0.21) and seems to be producedas a result of treatment with the antagonistic's, but does not appear in the control or in thestems and roots treated two water-peptone. The production of PR-proteins with β-1,3-glucanase activity presumably forms part of the hypersensitive defense mechanism ofpepper plants and would be responsible for the induction of the resistance developed bythe plant after treatment with the antagonists and infection by decapitation by P. capsici.
关键词：Isoenzymes;!!Pepper;!!Burkholderia cepacia;!!Trichoderma harzianum;!!Phytophthora capsici;!!Induction of resistance.