标题:Propagation of citrus rootstocks by stem cutting using auxin pretreatments: the case of citrumelo (Citrus paradisi Macf. x Poncirus trifoliata (L.) Raf.),
期刊名称:Journal of Materials and Environmental Science
印刷版ISSN:2028-2508
出版年度:2017
卷号:8
期号:11
页码:4085-4093
出版社:University of Mohammed Premier Oujda
摘要:In order to develop effective methods for rapid clonal multiplication of citrus rootstocks, we propose herein to investigate the effect of exogenously applied auxins on rooting and establishment of spring cuttings of two citrumelo accessions (Citrus paradisi Macf. x Poncirus trifoliata L. Raf), i.e. Swingle and Sacaton. Cuttings were taken from young greenhouse seedlings and exposed for 24 hours to different treatments including three auxin types (indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)) and two concentrations of each one (100 and 200 ppm). Treated cuttings were then raised along with control cuttings under greenhouse conditions for six weeks. The results taken at the end of this period have shown significant variations in response to auxin treatments regarding survival, rooting and sprouting parameters. These followed similar patterns for the two accessions and were found to be dependant on the nature of the auxin rather than its concentration, the genotype or the interaction of these factors. Among the different combinations tested, IBA at 100 ppm level gave the highest survival (100%), the highest rooting percentage (100%) and was selected as the most suitable for promoting sprouting of citrumelo cuttings. By contrast, the application of NAA gave poor results and exerted a particular inhibitory effect on initiation of Swingle citrumelo roots and sprouts.