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标题：Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial
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作者：Fabien Wauquier , Elsa Mevel , , Stephanie Krisa , Tristan Richard , Josep Valls , Ruth Hornedo-Ortega , Henri Granel , , Line Boutin-Wittrant , Nelly Urban , Juliette Berger , Stéphane Descamps , Jérôme Guicheux ,, , Claire S. Vinatier ,, , Laurent Beck ,, , Nathalie Meunier , Adeline Blot ; Yohann Wittrant
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2019
卷号：11
期号：12
页码：1-10
DOI：10.3390/nu11123071
出版社：MDPI Publishing
摘要：Polyphenols are widely acknowledged for their health benefits, especially for the prevention of inflammatory and age-related diseases. We previously demonstrated that hydroxytyrosol (HT) and procyanidins (PCy), alone or in combination, drive preventive anti-osteoathritic effects in vivo. However, the lack of sufficient clinical evidences on the relationship between dietary phytochemicals and osteoarthritis remains. In this light, we investigated in humans the potential osteoarticular benefit of a grapeseed and olive extract (OPCO) characterized for its hydroxytyrosol (HT) and procyanidins (PCy) content. We first validated, in vitro, the anti-inflammatory and chondroprotective properties of the extract on primary cultured human articular chondrocytes stimulated by interleukin-1 beta (IL-1 β). The sparing effect involved a molecular mechanism dependent on the nuclear transcription factor-kappa B (NF-κB) pathway. To confirm the clinical relevance of such a nutritional strategy, we designed an innovative clinical approach taking into account the metabolites that are formed during the digestion process and that appear in circulation after the ingestion of the OPCO extract. Blood samples from volunteers were collected following ingestion, absorption, and metabolization of the extract and then were processed and applied on human primary chondrocyte cultures. This original ex vivo methodology confirmed at a clinical level the chondroprotective properties previously observed in vitro and in vivo.
关键词：micronutrients; osteoarthritis (OA); clinical trials; hydroxytyrosol; procyanidins; cell biology micronutrients ; osteoarthritis (OA) ; clinical trials ; hydroxytyrosol ; procyanidins ; cell biology