文章基本信息

标题：Multiple physiological response analyses aid the understanding of sensitivity variation between Microcystis aeruginosa and Chlorella sp. under paraquat exposures
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作者：Fang Bai ; Yunlu Jia ; Cuiping Yang 等
期刊名称：Environmental Sciences Europe
印刷版ISSN：2190-4715
出版年度：2019
卷号：31
期号：1
页码：1-17
DOI：10.1186/s12302-019-0255-4
出版社：BioMed Central
摘要：Sensitivity differences to chemical pollutants in different phytoplankton species may potentially shape the community structure of phytoplankton. However, detailed information supporting the understanding of sensitivity variations between phytoplankton species is still limited. To investigate sensitivity differences between the cyanobacterium Microcystis aeruginosa, and the green alga Chlorella sp. to paraquat, multiple physiological parameters were measured and compared through acute and chronic toxicity assays. Early photosynthetic responses during acute toxicity assays showed that paraquat affects Photosynthesis System II energy fluxes in M. aeruginosa within 3 h of exposure, but not in Chlorella sp. After 5 h of cumulative exposure, an EC50 based on the maximum quantum yield for primary photochemistry of 0.54 mg L−1 was achieved and remained more or less constant, while the EC50 values for Chlorella fluctuated around 44.76 ± 3.13 mg L−1 after 24 h of exposure. During chronic 96 h exposure to paraquat, differences in antioxidant enzyme activities, reactive oxygen species (ROS) levels, and ultrastructure were observed in both M. aeruginosa and Chlorella sp. An increase in the intracellular levels of ROS and the number of plasma membrane damaged cells was observed in M. aeruginosa in the 0.2, 0.5, and 1.0 mg L−1 treatments (p < 0.01), but not for Chlorella. In addition, at an exposure level of 1.0 mg L−1, extensive disruption of cell structure was observed in M. aeruginosa. Conversely, little disarrangement of organelle structure was found in Chlorella sp. These results confirm that paraquat is more toxic to M. aeruginosa than to Chlorella sp. The sensitivity differences between these two species (one a prokaryote and the other a eukaryote) to paraquat might be partially explained by the differences in cell structure (cell wall and photosynthetic structure), the enzymatic antioxidant system, and the physiological vulnerability. The multiple physiological endpoint analysis approach used in the current study provides more detailed information for understanding the mechanisms of sensitivity variation between these phytoplankton species.
关键词：Early response;Chronic response;Growth inhibition;Photosynthesis-inhibiting herbicide;Phytotoxicity;Phytoplankton community