摘要:Background: The fresh herbs and spices used in Thai food are what make Thai cuisine
unique. Some of these herbs and spices have been scientifically studied for health benefits.
However, there is limited evidence for cooked food.
Objective: To determine proximate composition, fatty acid profile, phenolic and flavonoid
contents and antioxidant activity of fresh Massaman curry paste (MCP) and cooked
Massaman curry including beef and chicken Massaman curry (BMC and CMC, respectively).
Methods: The chemical composition and fatty acid profile of freeze-dried samples were
analyzed. Food samples were extracted by three solvents: water, absolute methanol or
ethanol. The total phenolic and flavonoid contents (TPC, TFC, respectively) of the extracts
were measured spectrophotometrically and identified through high performance liquid
chromatography (HPLC). The antioxidant activities of extracts were evaluated by 2,2-
dyphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing antioxidant power
(FRAP) assays.
Results: Carbohydrate, protein, and fat were the main components of all sample studies.
Chicken Massaman curry (CMC) contained highest fat and energy contents. Fatty acid profile
of CMC showed high content of lauric acid (9460 mg/100 g), oleic acid (6050 mg/100 g) and
palmitic acid (4220 mg/100 g). MCP showed high content of linoleic acid (213.70 mg/100 g)
and palmitic acid (195.71 mg/100g). Methanol extract of MCP showed the highest content of
TPC (1.076±0.013 mg gallic acid equivalent (GAE)/100g crude extract), TFC (27.10±0.31
mg CE/ 100g crude extract) and antioxidant activity by FRAP assay (0.311 ± 0.002 mg Trolox/100g crude extract). While ethanol extract of MCP showed the highest activity of
DPPH at value as 11.81±0.06 mg GAE/100 g crude extract, following by methanol extract of
MCP (11.02 ± 0.05 mg GAE/100 g crude extract). Cooked Massaman curry including BMC
and CMC showed low content of TPC and TFC and antioxidant than fresh MCP. Ferulic acid
was the main phenolic acid in these samples which was the highest in methanol extract,
followed by ethanol and then water extraction. After cooking process, some activities of food
were altered.
Conclusion: The results of this study suggest that fresh Massamun curry paste and cooked
Massuman curry in ready-to-eat foods could potentially show antioxidant activity. It provided
that the health benefits from plants source still remained in the products after cooking
process.