文章基本信息

标题：A unique variant of lymphocytic choriomeningitis virus that induces pheromone binding protein MUP: Critical role for CTL
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作者：Brian C. Ware ; Brian C. Ware ; Brian M. Sullivan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2019
卷号：116
期号：36
页码：18001-18008
DOI：10.1073/pnas.1907070116
出版社：The National Academy of Sciences of the United States of America
摘要：Lymphocytic choriomeningitis virus (LCMV) WE variant 2.2 (v2.2) generated a high level of the major mouse urinary protein: MUP. Mice infected with LCMV WE v54, which differed from v2.2 by a single amino acid in the viral glycoprotein, failed to generate MUP above baseline levels found in uninfected controls. Variant 54 bound at 2.5 logs higher affinity to the LCMV receptor α-dystroglycan (α-DG) than v2.2 and entered α-DG–expressing but not α-DG–null cells. Variant 2.2 infected both α-DG–null or –expressing cells. Variant 54 infected more dendritic cells, generated a negligible CD8 T cell response, and caused a persistent infection, while v2.2 generated cytotoxic T lymphocytes (CTLs) and cleared virus within 10 days. By 20 days postinfection and through the 80-day observation period, significantly higher amounts of MUP were found in v2.2-infected mice. Production of MUP was dependent on virus-specific CTL as deletion of such cells aborted MUP production. Furthermore, MUP production was not elevated in v2.2 persistently infected mice unless virus was cleared following transfer of virus-specific CTL..
关键词：pheromones ; MUP ; cytotoxic T lymphocytes (CTL) ; lymphocytic choriomeningitis virus (LCMV)