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标题：Effects of supplementation of Bacillus spp. on blood metabolites, antioxidant status, and gene expression pattern of selective cytokines in growing Barki lambs
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作者：Sabry Mousa ; Ahmed Elsayed ; Basma Marghani 等
期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2019
卷号：6
期号：3
页码：333-340
DOI：10.5455/javar.2019.f351
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objective: In this study, we investigated the potential immune-enhancing effects in addition to anti-oxidative stress properties of commercially accessible Bacillus subtilis supplementation in Barki lambs. Materials and Methods: Twenty apparently healthy weaned Barki lambs were used in this study and distributed randomly into two experimental groups: Negative control group, received control basal diet without any feed supplements and a supplemented group, received control basal diet supplemented with water added to commercially accessible bacilli at 1 gm/l/day for 30 consecutive days. Blood samples were collected from each lamb before starting the experiment (T0), 2 weeks (T15), and 4 weeks (T30) post-supplementation for serum biochemical analyses, total leucocytes and lymphocytes count, and real-time polymerase chain reaction assays. Results: The supplemented group showed a significant increase ( p < 0.05) in the total number of leukocytes and the number of lymphocytes, lysozyme activity, reduced glutathione, total antioxidant capacity with a significantly lower malondialdehyde values at T30 and significantly higher levels ( p < 0.05) of serum catalase and nitric oxide at T15 as compared with control ones. B. subtilis elicited maximal up-regulation of most of the studied genes compared with the control group. Conclusion: The results herein suggest that B. subtilis could be used as useful nutritional supplements to support the immune system in healthy lambs.
关键词：Bacillus subtilis; immunity; antioxidant status; RT-PCR; sheep