文章基本信息

标题：A Probe for Fluorescence Detection of the Acetylcholinesterase Activity Based on Molecularly Imprinted Polymers Coated Carbon Dots
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作者：Zhao Jia ; Yane Luo ; Huiyun Wen 等
期刊名称：Chemical and Pharmaceutical Bulletin
印刷版ISSN：0009-2363
电子版ISSN：1347-5223
出版年度：2019
卷号：67
期号：8
页码：795-800
DOI：10.1248/cpb.c18-00944
出版社：The Pharmaceutical Society of Japan
摘要：This paper presents a new probe for fluorescence detection of the acetylcholinesterase (AChE) activity based on molecularly imprinted polymer (MIP) coated carbon dots (C-dots) composite. The C-dots were hydrothermally synthesized with grafted silica surface and sealed with molecularly imprinted polymers in silica pores (MIP@C-dots) in situ . Removed the original template molecules, the MIP@C-dots composite exhibits quite high selectivity for acetylthiocholine (ACh). With AChE, its substrate ACh will be hydrolyzed into thiocholine and the fluorescence signals exhibit a dramatic decrease at 465 nm, Under optimal conditions, the fluorescent probe shows sensitive responses to AChE in the range of 0.01–0.6 mU/mL. The detection limits of AChE are as low as 3 µU/mL. These experiments results validate the novel fluorescent probe based on MIP@C-dots composite, paving a new way to evaluation of AChE activity and Screening inhibitors.
关键词：acetylcholinesterase;fluorescence probe;surface imprinting;carbon dot