文章基本信息

标题：Sponge-associated fungi from a mangrove habitat in Indonesia: species composition, antimicrobial activity, enzyme screening and bioactive profiling
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作者：Mada Triandala Sibero ; Yasuhiro Igarashi ; Ocky Karna Radjasa 等
期刊名称：International Aquatic Research
印刷版ISSN：2008-6970
出版年度：2019
卷号：11
期号：2
页码：173-186
DOI：10.1007/s40071-019-0227-8
出版社：BioMed Central
摘要：There is no report of diversity, biological properties and bioactive compounds of sponge-associated fungi from Indonesia’s mangrove ecosystem to date. This study was designed to isolate sponge-associated fungi from a mangrove ecosystem in Mangkang, to screen the antimicrobial and extracellular enzyme properties of the isolates, characterize the biologically promising isolates using molecular approaches, and profile the secondary metabolites using phytochemical and thin-layer chromatography (TLC) analyses. An unidentified sponge that lived in association with mangrove roots was collected from Mangkang. Total of eight associated fungi were isolated from the sponge. Among all isolates, only two fungi SPMKF 1 and SPMKF 6 produced extracellular amylase, another two fungi SPMKF 4 and 5 showed antibacterial activity against MRSA, and only one fungus SPMKF 8 was able to produce extracellular amylase and show antimicrobial activity against ESBL E . coli , Salmonella enterica ser. Typhi strain MDR and C . albicans , while SPMKF 2, SPMKF 3 and SPMKF 9 did not show any biological properties. The result of genetic characterization proved that SPMKF 1 was Cladosporium tenuissimum , SPMKF 4 was Eutypella sp., SPMKF 5 was Lasiodiplodia theobromae , SPMKF 6 was Fusarium keratoplasticum and SPMKF 8 was F . solani . Furthermore, an amylase gene was detected in fungi SPMKF 1, 6 and 8 while among the BGC, only NRPS genes were detected in SPMKF 4, 5 and 8. Interestingly, several same metabolites indicating the same retention factor ( R f) values in TLC were detected in the fungal crude extracts by TLC.
关键词：Fungi ; NRPS ; Sponge ; Mangrove ecosystem