文章基本信息

标题：Optimization of Hydrolyzed Crude Extract from Tartary Buckwheat Protein and Analysis of Its Hypoglycemic Activity in Vitro
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作者：Ting Tao ; Di Pan ; Ya Ying Zheng 等
期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：295
期号：3
页码：1-9
DOI：10.1088/1755-1315/295/3/032065
出版社：IOP Publishing
摘要：Tartary buckwheat was used as substrate for enzymatic hydrolysis to prepare a crude extract of tartary buckwheat protein, hydrolyzed by alcalase to simulate digestion in vivo. On the basis of single factor experiment, buckwheat protein hydrolysis was served as the response value. By Box-Behnken experimental, the optimum conditions were determined as volume of enzyme 40.61 U/g, temperature 46.16°C, time 20.80 min. Under these conditions, the actual degree of hydrolysis of the tartary buckwheat protein could reach to 57.71%, in good agreement with the predicted value (57.69%), and the inhibition of α-amylase activity was 78.69%, respectively.