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标题：The effects of lead on ATPase activity in liver of Pelophylax nigromaculata
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作者：Minyi Huang ; Jinjin Ma
期刊名称：IOP Conference Series: Earth and Environmental Science
印刷版ISSN：1755-1307
电子版ISSN：1755-1315
出版年度：2019
卷号：252
期号：4
页码：1-6
DOI：10.1088/1755-1315/252/4/042065
出版社：IOP Publishing
摘要：Lead is a major heavy metal in sewage, which affects the growth, development, reproduction, endocrine and blood circulation of aquatic animals. The effects of lead on the activity of ATPase in liver of Pelophylax nigromaculata were studied. In vitro experiment, the grinding fluid of the liver of P. nigromaculata was mixed with the equal proportion of 0, 0.1, 10, 100, 1000 and 10000 ug/L lead. In vivo experiment, the liver was treated with 0, 10, 100 and 1000 g/L of lead for 30 d. The absorbance of the liver was measured by ATPase kit with enzyme labeling instrument and ATPase activity of the liver was calculated according to the formula. In vitro experiments, compared with the control group, the activity of ATP enzyme in lead treated group decreased significantly (p<0.05). With the increase of lead concentration, the activity value showed a fluctuating trend of first decreasing and then rising and then falling, and the activity value of 10 μg/L ATP in treatment group decreased most (p<0.05). With the increase of Pb concentration, the activity of ATP decreased first and then increased, and there was a significant difference among the treatment groups (p<0.05). The activity of 100 μg/L ATP decreased most in the treatment group. The results showed that lead treatment inhibited the activity of ATP enzyme in the liver of P. nigromaculata.